{"created":"2024-07-01T02:24:54.781264+00:00","id":2000017,"links":{},"metadata":{"_buckets":{"deposit":"19e5e64e-3099-47e6-83be-b28ba71002b9"},"_deposit":{"created_by":14,"id":"2000017","owner":"14","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"2000017"},"status":"published"},"_oai":{"id":"oai:nagoya-wu.repo.nii.ac.jp:02000017","sets":["239:1718934816370"]},"author_link":[],"control_number":"2000017","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-10"},"bibliographicIssueNumber":"70","bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"名古屋女子大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of Nagoya Women's University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ASF-4 series of cells were established from tissues collected from a single Japanese individual over the course of 40 years. Previously, we reported that the replicative lifespan of ASF-4 cells was reduced and was dependent on the increasing age of the donor as the telomere length was shortened. In this study, using ASF-4 cells that included cells from donors aged 60 years or older, we confirmed that the replicative lifespan of the cells was negatively correlated with donor age. We created a mathematical model to estimate the replicative lifespan from the experimental data and showed that the replicative lifespan can be determined with high accuracy. Furthermore, we found that the variation in the replicative lifespan increased with a donor age above 60 years, the correlation with donor age was lower, and the parameters of the mathematical model showed a greater variation in cells from a donor aged 60 years or older. These results confirmed that the replicative lifespan of cells decreases depending on the individual’s age and suggested that, in the case of humans, there is more diversity in the phenomenon of cellular aging for individuals older than 60 years.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"ASF-4系列の細胞は、単一の個人から40年にわたり、５−10年間隔、提供年齢で、36歳から76歳にわたり採取された組織から作られた細胞である。以前、我々はこの細胞系を用いて、提供年齢に応じて細胞の分裂寿命が短縮していること、テロメア長が短縮していることなどを報告した。本研究では、以前の報告後に得られた60歳以上の提供年齢の細胞を含むASF-4系列の細胞を用いて、提供年齢と細胞の分裂寿命が負の相関性を示すことを確認するとともに、実験データから分裂寿命を推定する数理モデルを作成し、再現性良く分裂寿命が求められることを示した。さらに、60歳以上の提供年齢では、分裂寿命についてのばらつきが大きくなり、提供年齢との相関性が低くなること、また数理モデルのパラメーターも60歳以上の提供年齢の細胞でばらつきが大きくなることを見出した。以上から、Hayflickらの提唱した、個体老化により細胞の分裂寿命が減少することを再度確認するとともに、ヒトの場合は、60歳を超える個体について細胞の老化現象に多様性が顕著にあらわれることを示唆する結果を得た。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"名古屋女子大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Nagoya Women's University","subitem_publisher_language":"en"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"24368830","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nobuyuki, HORIE","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kazuhiko, KAJI","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-07-01"}],"displaytype":"detail","filename":"kiyo70_1-11.pdf","filesize":[{"value":"507 KB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"url":"https://nagoya-wu.repo.nii.ac.jp/record/2000017/files/kiyo70_1-11.pdf"},"version_id":"0c05fc7a-88e7-45bb-9df7-e8c406a596a0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"individual aging","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cellular senescence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"replicative senescence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"telomere","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Re-evaluation of the Relationship between Donor Age and Replicative Lifespan Using ASF-4 Series of Fibroblasts Established from a Single Individual : Application of a Mathematical Model and Examination of Aging Parameters","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Re-evaluation of the Relationship between Donor Age and Replicative Lifespan Using ASF-4 Series of Fibroblasts Established from a Single Individual : Application of a Mathematical Model and Examination of Aging Parameters","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"14","path":["1718934816370"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-01"},"publish_date":"2024-07-01","publish_status":"0","recid":"2000017","relation_version_is_last":true,"title":["Re-evaluation of the Relationship between Donor Age and Replicative Lifespan Using ASF-4 Series of Fibroblasts Established from a Single Individual : Application of a Mathematical Model and Examination of Aging Parameters"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-07-01T02:38:05.176650+00:00"}