@article{oai:nagoya-wu.repo.nii.ac.jp:00004313,
 author = {堀江, 信之 and 高橋, 和利 and 加治, 和彦},
 issue = {65},
 journal = {名古屋女子大学紀要. 家政・自然編, 人文・社会編},
 month = {Mar},
 note = {We performed Epigenetic Clock（EC） analysis of ASF-4 cells, which were derived from a
single donor at different ages over 30 years. Optimization using a function of MethylAger
software was performed and we demonstrated that it is possible to estimate the ages from
methylation sites for both ASF-4 cells and iPS cells prepared from ASF-4 cells. For ASF-
4 cells, iPS cells, and all the cells, we were able to select three to eight methylation sites,
from which the donor ages were estimated with a root mean squared error of fewer than 6
years. Methylation sites obtained by analysis of EC tend to exist in the vicinity of genes, and
these genes contained genes related to aging that have been reported so far. Since it was
possible to estimate age not only for fibroblasts but also for iPS cells derived from them,
it is suggested that some age-dependent methylation sites exist even in the iPS cells. It is
also suggested that analysis of the epigenetic clock is valuable as a method to identify genes
related to aging involving DNA methylation.},
 pages = {27--38},
 title = {個人の組織から分離された繊維芽細胞及びそれらから作成されたiPS細胞のエピジェネティッククロック解析},
 year = {2019}
}