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セルロースアセテート膜電気泳動法による血清リポタンパク質の分析条件の検討
https://nagoya-wu.repo.nii.ac.jp/records/4306
https://nagoya-wu.repo.nii.ac.jp/records/43069f6e7ff4-8f66-49d3-8e0d-68e66d98f1b2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kojnkasei64_39-45 (416.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2018-06-28 | |||||||
| タイトル | ||||||||
| タイトル | セルロースアセテート膜電気泳動法による血清リポタンパク質の分析条件の検討 | |||||||
| タイトル | ||||||||
| タイトル | Separation and Detection of Serum Lipoproteins by Electrophoresis on Cellulose Acetate Membrane | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 著者 |
堀江, 信之
× 堀江, 信之
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Electrophoresis using cellulose acetate membranes as support media is widely used for clinical examination of serum protein. In this study, analytical conditions of serum lipoprotein with cellulose acetate electrophoresis were investigated. Similar to the analysis of serum protein by cellulose acetate electrophoresis, a good separation and stained image was obtained by cellulose acetate electrophoresis using sodium barbital buffer solution and staining with Sudan Red 7B. Based on the measurement of the density of the bands of α- and β-lipoprotein fractions including HDL and LDL, respectively, the α- and β-lipoprotein fraction ratio（α/β ratio）was calculated. This value was found to be close to the HDLcholesterol / LDL-cholesterol value, which is attracting attention as a clinical test value related to a risk factor for sudden cardiac death. Because analysis of serum lipoprotein by cellulose acetate electrophoresis established in the report can be performed with as little as 2μL of human serum sample and the whole procedure can be completed within 2 hours, it is considered to be a valuable program for an experimental course in medical education. |
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| 書誌情報 |
名古屋女子大学紀要. 家政・自然編, 人文・社会編 号 64, p. 39-45, 発行日 2018-03 |
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| 出版者 | ||||||||
| 出版者 | 名古屋女子大学 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 2185-7962 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA12530109 | |||||||
